Research Technician III - Biochemistry - Molecular Biology
There are different opportunities in Bernadette Holdener's Lab- Please review and apply individually to the position(s) of interest, to be considered an applicant for each specific position.
Required Qualifications: (As evidenced by an attached resume)
Degree (equivalent) or higher with a concentration in biology or related field. Two year's of full-time experience in a cell/molecular biology research lab setting. Experience localizing proteins in tissue sections or embryos using immunohistochemical and immunofluorescence staining. Experience with light and fluorescent microscopy and digital imaging.
Experience embedding and sectioning tissues (paraffin, cryo, and/or vibratome). Experience with confocal microscopy or similar. Experience analyzing gene expression using in situ hybridization and/or quantitative RT-PCR. Experience with mouse husbandry, euthanasia, and anesthesia procedures.
Brief Description of Duties:
Under direction of the PI, the Research Technician III will assist with experiments focused on understanding how defects in glycosylation lead to birth defects in brain, skeleton, eye (anterior chamber defects). The primary focus will be determining whether the mutations affect protein trafficking and/or alter extracellular matrix composition/properties during organ development. The successful incumbent must have strong organizational and time management skills with exceptional attention to detail. The degree to which incumbent has experience with the design, implementation, and troubleshooting of experiments. Experience successfully working as well as part of a team. Outstanding written, verbal, and interpersonal communication skills. Meticulous record keeping.
- Working under general supervision, assist in research program to localize proteins and RNA in sectioned adult and embryonic tissues or by Western analysis and to localize and quantitate mRNA in whole mount or sectioned embryos and adult tissues.
- Compile scientific data/results from highly technical experiments and report to PI. Participate in weekly meetings with co-PI lab.
- Assist with mouse Husbandry and lab maintenance and organization.
- Train Graduate and Undergraduate students/trainees in cell and molecular biology techniques.
- Other duties or projects as assigned.
About Stony Brook:
Stony Brook University, home to many highly ranked graduate research programs, is located 60 miles from New York City on Long Island's scenic North Shore. Our 1,100-acre campus is home to 24,000 undergraduate, graduate, and doctoral students and more than 13,500 faculty and staff, including those employed at Stony Brook Medicine, Suffolk County's only academic medical center and tertiary care provider. The University is a member of the prestigious Association of American Universities and co-manager of nearby Brookhaven National Laboratory (BNL), a multidisciplinary research laboratory supporting world class scientific programs utilizing state-of-the-art facilities such as the Relativistic Heavy Ion Collider, the National Synchrotron Light Source, and the Center for Functional Nanomaterials, and the New York Blue IBM BG/L+P supercomputer, owned by Stony Brook and managed by BNL. Stony Brook is a partner in managing the Laboratory for the Department of Energy, and is the largest institutional scientific user of BNL facilities. As such, many opportunities exist for collaborative research, and in some cases, joint appointments can be arranged.
Equal Opportunity Employer, females, minorities, disabled, veterans.
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